[Lotus]

Well, I guess building breast tissue means binding estrogen receptors in the breast. Maybe this has nothing to do with the estrogen peak in the bloodstream, besides all hormones are likely to be pulsating, may be being high estrogen all the time doesn't help.

...and I had a thought last night. Estrogen injections aren't time-release, are they? That "peak" is ok, so why wouldn't this be? You know whose input I'd love to get on this? Lotus's.

Hey there Nipply. 



MTF TRANS HORMONAL THERAPY
HRT. Options, Best Practices, and some other semi-related stuff…
https://moderntranshormones.com/2017/11/...estrogens/



From my experience IM (intramuscular) worked the best for me. I would take a .25 ml injection per week of EV (estradiol valerate). Believe or not there's a micronized rectal estradiol suppository. Pueraria mirifica needs liver activation for the chromene compound miroestrol/deoxymiroestrol to become active...it has to activate certain enzymes to become biologically active. 


Requirement of metabolic activation for estrogenic activity of Pueraria mirifica
Y S Lee et al. J Vet Sci. 2002 Dec.

Abstract
A wide range of chemicals derived from plant and human-made xenobiotics are reported to have hormonal activities. The present study was performed to examine the estrogenic effect of Kwao Keur, Pueraria mirifica (PM), that has been used as a rejuvenating folk medicine in Thailand, using recombinant yeast, MCF-7 cell proliferation and HepG2 cell transient transfection assay. In recombinant yeast assay, 0.025, 0.25, 2.5, 25, 2.5 x 10(2), 2.5 x 10(3), 2.5 x 10(4) ng/ml concentrations of PM did not show any estrogenic activities, while 10(-9) of 17 beta-estradiol (positive control) showed high estrogenic activity. Estrogenic activities were induced at 2.5 ng/ml to 25 microg/ml concentrations of PM in a dose-dependent manner on MCF-7 cells and the estrogenic effect of PM was blocked by tamoxifen treatment, a well-known anti-estrogen. PM also showed estrogenic effect on human hepatoma cell line, HepG2 cells, containing estrogen receptor and luciferase reporter gene. Taken together, PM in itself may have no estrogenicity in yeast system, but it has estrogenicity in MCF-7 & HepG2 cells that have human metabolic enzymes. The results indicated that PM may require metabolic activation for estrogenic activity.

And also was reported here:

Pueraria mirifica. Metabolic activation promotes estrogenic activity.
https://www.breastnexum.com/showthread.php?tid=20349&page=2&highlight=Activation

Have you tried a rectal suppository method?. Coconut oil or olive oil (heated) make decent carrier oil for suppositories. Just mix the PM in the heated oil and pour into a suppository mold and freeze. Then upon administration, put a dab of oil on the tip and insert. Use finger cots or disposable vinyl gloves. See, the aqueous version (warm water w/pm) may not pass into the digestive tract as effectively as PM w/a carrier oil. Meaning the carrier oil (fats) diffuse easier over the cell membrane (receptor)...in fact, one should take NBE with an oil. So, I think you're wanting a slower release that has higher bioavailability...try using the suppository administration and see if it works out.


Absorption from aqueous and alcoholic solutions may occur very rapidly, which has proved to be of considerable therapeutic value in the rapid suppression of acute convulsive attacks by diazepam (e.g. in children), but absorption from suppositories is generally slower and very much dependent on the nature of the suppository base, the use of surfactants or other additives, particle size of the active ingredient, etc.

Rectal drug administration: clinical pharmacokinetic considerations
https://pubmed.ncbi.nlm.nih.gov/6126289/