(23-03-2016, 04:52 PM)Dianna1395 Wrote: (09-03-2016, 04:59 AM)Lotus Wrote: (09-03-2016, 03:50 AM)MarcyAno Wrote: That date is in the Buddhist calendar format used in Thailand. I looked it up. I think it translates to 2014. Its definitely not 1958 I'll do some more digging on the other details and report back.
Super, I'd love to hear the feedback. Ever wonder how (or in what strength) Phytoestrogen bind to receptors?, well, I do lol.
Phytoestrogens modulate binding response of estrogen receptors alpha and beta to the estrogen response element.
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Kostelac D1, Rechkemmer G, Briviba K.
Author information
Abstract
Binding of estrogen receptor (ER) to estrogen response element (ERE) induces gene activation and is an important step in estrogen-induced biological effects. Here, we investigated the effects of some dietary phytoestrogens such as the isoflavones genistein and daidzein, its metabolite equol, and the coumestane coumestrol on the binding rate of ERalpha and ERbeta to ERE by a nonradioactive real-time method, the Biacore Technology. ERalpha and ERbeta were able to bind to ERE immobilized on the surface of a sensor chip even in the absence of estrogens. 17beta-Estradiol and phytoestrogens induced an increase in ER binding to ERE in a concentration-dependent manner. 17beta-Estradiol was a more potent activator of binding than the phytoestrogens studied. The concentrations of 17beta-estradiol inducing an increase in the binding response of ERalpha and ERbeta to ERE by 50% (EC(50)) as compared to unliganded ER were 0.03 and 0.01 microM, respectively. Regarding the efficacy of activation of ERalpha, from the most to the least effective compound, the sequence and the EC(50) were as follows: 17beta-estradiol (0.03 microM) > coumestrol (0.2 microM) > equol (3.5 microM) > genistein (15 microM) > daidzein (>300 microM) and for ERbeta 17beta-estradiol (0.01 microM) > coumestrol (0.025 microM) > genistein (0.03 microM) > daidzein (0.35 microM) > equol (0.4 microM). The ratios EC(50)alpha/EC(50)beta were calculated to be for 17beta-estradiol, 3; coumestrol, 8; equol, 8.8; genistein, 500; daidzein > 850. These ratios indicate that genistein and daidzein preferentially activate the binding of ERbeta to ERE. The endogenous hormone 17beta-estradiol as well as coumestrol and daidzein metabolite equol activate the binding of ERbeta to ERE only slightly more effectively than the binding of ERalpha to ERE. Thus, the effect of daidzein can be changed from a specific activator of ERbeta to an activator of both ER isotypes alpha and beta in humans who are able to convert daidzein to equol. While the results of the measurements with ERalpha were in line with the binding affinities of compounds tested for ER, there was a distinct difference between our results and the binding affinities of phytoestrogens for the ERbeta. This leads to the conclusion that phytoestrogens differ not only in their binding affinities for the ER, but also in their potential to increase the rate of receptor binding to the ERE.
Hi, Lotus.
Trying to sort through a lot, but.... This would again imply that for development, meaning fast and effective development, the Pharma route is still "better." Even lower-cost.
So, to get the best mix...
Maybe pharma for growth first (plus massage for the benefits it will bring); then, for maintenance, maybe use our established Phytoestrogens? I.E., take a hit to the liver up front, and get the development; then, go to PM and/or BO, and keep the development that way, while we also cleanse the liver.
Any thoughts on that as a concept?
-Dianna
Fast metabolism is what makes sense to me, why give estradiol any more chances (ideas lol) to hang around, the bad metabolites alone can ruin a good day at the office (aka the liver). Which the liver tells us (in many ways) it really prefers a faster synthesis of hormones, and the liver is huge at processing E2 (like 90% of it). Too bad only 10-15% gets through (herbal/drug supplementation), what a waste lol. Imo sublingual/transdermal yields a faster feminization, or liquor is quicker (as the saying goes), suppose that's why male alcoholics feminize their livers faster.
The only other argument to be made is IM delivery, a steady amount (or dose dependent is delivered (patch too). From what I've seen, personally I really do believe sublingual sees a faster target tissue delivery, what that's?, aka breasts, (or organs, cells with a specific response from hormones), others will undoubtedly disagree, that's fine, I'm cool with dat too
. I'm not for changing people's religion on NBE. Lol, infact, I don't know why I hang around you people anyways,
, (kidding, you know I love you guys).