[tanysquirrel]

Some thoughts on turning off the amh gene:

From above: "Expression of AMH is activated by SOX9" If you turn off SOX9 would it not deactivate the AMH?

See Figure 3 from this article - http://onlinelibrary.wiley.com/doi/10.10...900193/pdf 

According to this article the FOXL2 gene suppresses the SOX9 gene. From some reading I did in the past, FOXL2 is found in the ovaries of all mammalian vertebrate species so by my thinking, this should be found in cow ovaries. So if one were to smear some sort of Bovine Ovary cream on their boys... Oh wait, that's what I've been doing for the past few weeks. I'm not sure whether that would be enough to flip that testes to ovary switch, but I might just find out.

I don't think you would be able to switch ducts though, seems to me the Müllerian duct is destroyed by AMH is it not?

Anyway, interesting articles, thanks for posting.

according to this paper, it IS found in bovine ovaries: 

https://www.karger.com/Article/Pdf/447611

 "This generated a scenario where Foxl2 is predominantly expressed in ovarian somatic cells and Foxl3 in male germ cells. To support this hypothesis, we provide original results showing that in the pea aphid (insects)  foxl2/3  is predominantly expressed in sexual females and showing that in bovine ovaries  FOXL2  is specifically expressed in granulosa cells. Overall, current results suggest that Foxl2 and Foxl3 are evolutionarily conserved players involved in somatic and germinal differentiation of gonadal sex." ©

Furthermore:
Mutations in the  FOXL2  gene were found to be responsible for blepharophimosis-ptosis-epicanthus syndrome (BPES, OMIM #110100) in humans, a condition involving eyelid malformations and premature loss of ovarian function [Crisponi et al., 2001].  FOXL2  was initially named  PFRK  for pituitary forkhead factor based on its first identification as a gene expressed in the pituitary [Kioussi et al., 1999]. In this tissue,  FOXL2  was found to be expressed in all gonadotropes and thyrotropes and a small fraction of prolactin-containing cells during pregnancy, but not in somatotropes or corticotropes. As first demonstrated in the above-mentioned BPES phenotype, FOXL2 is also involved in cranio-facial development [Crisponi et al., 2001]. In BPES in humans,  FOXL2  haploinsufficiency leads to eyelid malformation [Beysen et al., 2008], but its total loss of function (complete knockout leading to homozygous null mutations) in mice [Schmidt et al., 2004; Uda et al., 2004] and goats [Boulanger et al., 2014] results in a complete absence of eyelids. Moreover, in cranio-facial development,  Foxl2  is involved not only in eyelid differentiation but also in extraocular muscle and bone differentiation [Heude et al., 2015].  Foxl2  is also expressed both by cranial neural crest cells (CNCCs) and by cranial mesodermal cells (CMCs), which give rise to skeletal (CNCCs and CMCs) and muscular (CMCs) components of the head.  Foxl2  conditional inactivation in mice, in either CNCCs or CMCs, shows that FOXL2 function in CNCCs is necessary for the development of the levator palpabrae superioris, the superior and inferior oblique muscles of the eyelid.  Foxl2  deletion in either CNCCs or CMCs prevents eyelid closure and induces subtle skeletal developmental defects [Heude et al., 2015]. In addition, FOXL2 is also involved in cartilage and skeletal formation, bone mineralization, and growth as demonstrated in a constitutive  Foxl2- deficient mice model [Shi et al., 2014; Marongiu et al., 2015].  The first demonstration of the involvement of  FOXL2  in ovarian development was shown in type I BPES where affected women suffer not only from eyelid malformation but also from premature ovarian failure [Crisponi et al., 2001]. At about the same time, FOXL2 was also shown to be involved in the polled intersex syndrome in goats, where a natural deletion of 300 kb containing the  FOXL2  gene and 3 long non-coding RNAs leads to the extinction of  FOXL2  ovarian expression and triggers early testis differentiation and XX female-to-male sex reversal"

Oddly,  when I last went for an eye exam, they had diagnosed me with Blepheritis..

Be sure to read the whole article, there is too much to copy and paste on here.