14-04-2015, 01:11 AM
That's nice work lovely, however the bottom line is that fenugreek is still and always will be a phytoestrogen.
In vitro estrogenic activities of fenugreek Trigonella foenum graecum seeds
DLR assay measures estrogen activity through binding of estrogen receptor (ER α) in MCF-7 cells transiently transfected with a plasmid coding estrogen response elements with a reporter gene (ERE-luc) and a plasmid coding for ERα. FCE activated the expression of estrogen responsive reporter genes in MCF-7 cell lines while estrogen antagonists like tamoxifen downregulated the effects. In MCF-7 cells transfected with ERα and ERE-luc there was an upregulation of luciferase transcription after treatment with FCE and this was comparable with that of 17- β estradiol treatment. In the presence of an agonist, the ER initiates transcriptional activation by binding to specific EREs in the promoters of target genes23.
http://icmr.nic.in/ijmr/2010/june/0613.pdf
In vitro estrogenic activities of fenugreek Trigonella foenum graecum seeds
DLR assay measures estrogen activity through binding of estrogen receptor (ER α) in MCF-7 cells transiently transfected with a plasmid coding estrogen response elements with a reporter gene (ERE-luc) and a plasmid coding for ERα. FCE activated the expression of estrogen responsive reporter genes in MCF-7 cell lines while estrogen antagonists like tamoxifen downregulated the effects. In MCF-7 cells transfected with ERα and ERE-luc there was an upregulation of luciferase transcription after treatment with FCE and this was comparable with that of 17- β estradiol treatment. In the presence of an agonist, the ER initiates transcriptional activation by binding to specific EREs in the promoters of target genes23.
http://icmr.nic.in/ijmr/2010/june/0613.pdf